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Tajrin, Tania, 2011. Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger. First cycle, G2E. Uppsala: SLU, Dept. of Microbiology

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Abstract

There are several fungal species including Aspergillus niger that are responsible for food
spoilage. The conidia of Aspergillus niger contain mannitol and trehalose and these
compounds act as stress protectants and keep the cells viable. In my experiments, I
observed the survival after stress in trehalose and mannitol deficient mutants of
Aspergillus niger and compared with wild type strains. I applied different treatments like
heat shock, freeze-drying and different energy sources on mutants and wild types spores
at different ages. The results indicate that 1-month-old spores of mutant of ΔtppB and
ΔmpdA are more vulnerable at high and low temperatures. These experiments indicate
that the trehalose deficient mutant ΔtppB and the mannitol deficient mutant ΔmpdA are
more sensitive compared to wild type and most probably due to their lower mannitol and
trehalose levels.

Main title:Stress tolerance and growth of trehalose and mannitol deficient mutants in Aspergillus niger
Authors:Tajrin, Tania
Supervisor:Melin, Petter
Examiner:Leong, Su-lin
Series:Independent project / Swedish University of Agricultural Sciences, Department of Microbiology
Volume/Sequential designation:2011:5
Year of Publication:2011
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:None
Department:(NL, NJ) > Dept. of Microbiology
Keywords:Aspergillus niger, mannitol, trehalose
URN:NBN:urn:nbn:se:slu:epsilon-s-63
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-63
Subjects:Food science and technology
Food contamination and toxicology
Language:English
Deposited On:19 Apr 2011 07:34
Metadata Last Modified:20 Apr 2012 14:18

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