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Kibria, Khairul, 2021. Lipid content and profile of Rhodotorula toruloides during fermentation. Second cycle, A2E. Uppsala: SLU, Department of Molecular Sciences



Oleaginous yeast such as Rhodotorula toruloides is a promising organism for production of lipids from lignocellulosic substrates. Microbial lipids have potential applications in energy, food, feed and pharmaceutical industry, because most of the lipids are triglycerides with long chain fatty acids that are comparable to typical vegetable oils and can be obtained without arable land requirement. This study investigated lipid production in the batch cultivation over time by the yeast Rhodotorula toruloides. Furthermore, the composition of fatty acids and lipid classes were analyzed by using gas chromatography (GS) and thin layer chromatography (TLC), respectively. Under the batch cultivation over time, maximum lipid content 34% of the cell dry weight was obtained on the day 5 and 6. However, interestingly polyunsaturated fatty acid (PUFA), omega-3 fatty acid (n-3) and omega-6 fatty acid (n-6) compositions on day 6 is higher than day 5. Through analyzing of total lipid compositions and TAG analysis, the most abundant fatty acids are oleic acid (C18:1), palmitic acid (C16:0) and linoleic acid (C18:2), which are useful precursors for conversion to biofuel production.
A higher n-6/n-3 ratio is observed during total lipid and triglyceride analysis, however, a lower ratio is advantageous in order to avoid negative impacts on human or animal health, if the oil is used for food or feed. The potentialities of lipids accumulation on shorter culture time and culture conditions are the key elements for the success of scale-up and profitability bioprocess.

Main title:Lipid content and profile of Rhodotorula toruloides during fermentation
Authors:Kibria, Khairul
Supervisor:Pickova, Jana
Examiner:Sampels, Sabine
Series:Molecular Sciences
Volume/Sequential designation:2021:08
Year of Publication:2021
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM003 Biotechnology - Master's Programme 120 HEC
Supervising department:(NL, NJ) > Department of Molecular Sciences
Keywords:Rhodotorula toruloides, fatty acids, lipid class, triglycerides, phospholipid, gas chromatography
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Subject. Use of subject categories until 2023-04-30.:Food science and technology
Deposited On:19 May 2021 11:28
Metadata Last Modified:20 May 2021 01:00

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