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Zetterlind, Bobby, 2020. Isolation and cultivation of methanogenic archaea from serpentinization system at Chimaera Seeps, Turkey. First cycle, G2E. Uppsala: SLU, Department of Molecular Sciences


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Abstract

A methanogenic archaeon was successfully isolated from iron-rich environmental samples from the Chimaera seeps in Turkey by cultivation in anoxic media supplemented with H2/CO2 and an antibiotic, namely erythromycin. The morphological features of this methanogen were non-motile rod cells approximately 1.7 – 8.5 μm in length and 0.3 – 0.4 μm in width and occurred most frequently as singular cells or aggregated in small clusters but were also observed in filamentous structure. The morphology corresponds with the species Methanobacterium oryzae found in rice fields in Philippines. Moreover, based on 16S rRNA gene sequence, this isolate in earlier studies showed 98.6 % sequence similarity to this M. Oryzae species. In similarity to this species, the obtained isolate from this study showed the ability to utilise H2 as electron donor and CO2 as carbon source to produce CH4, which also simulates the energy resources at the onshore low-temperature serpentinization system at the Chimaera seeps in Turkey. Hence, this study proposes that the Chimaera site can hosts hydrogenotrophic methanogens and provides with a biotic influx of CH4 and not only abiotically produced CH4. Besides, this study also suggests that there might exist a correlation between iron-rich sites and the isolated methanogen.

Main title:Isolation and cultivation of methanogenic archaea from serpentinization system at Chimaera Seeps, Turkey
Authors:Zetterlind, Bobby
Supervisor:Westerholm, Maria
Examiner:Schnürer, Anna
Series:Molecular Sciences
Volume/Sequential designation:2020:13
Year of Publication:2020
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:Other
Supervising department:(NL, NJ) > Department of Molecular Sciences
Keywords:Archaea, methanogens, Chimaera seeps, serpentinization system, isolation, cultivation
URN:NBN:urn:nbn:se:slu:epsilon-s-16000
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-16000
Subjects:Nature conservation and land resources
Language:English
Deposited On:17 Sep 2020 08:13
Metadata Last Modified:18 Sep 2020 01:01

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