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Elander, Pernilla, 2015. Towards the identification of Tudor SN interactome : cloning of putative interactors. First cycle, G2E. Uppsala: SLU, Department of Plant Biology (from 140101)

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Abstract

Tudor staphylococcal nuclease (TSN) is a protein found associated with stress granules (SG) and processing bodies (PB) where it acts as a part of the plant stress response. In plants not much is known for TSN. This project serves as a starting point in our attempt to elucidate the physically interacting proteins of TSN. Previously, our group identified proteins that can be physical interactors of TSN. Herein, we focused on TCTP, MAPK, Armadillo, FY, DHH1 and Kin11. By using overlay PCR, I fused the cDNAs of these genes with their corresponding promoters, and by using the Gateway technology I finally introduced these fusions to a binary vector which contains the protein fluorescent marker GFP. These binary vectors were transformed into Agrobacterium tumefaciens. In parallel, the promoters of these genes were introduced into a binary vector upstream of a uidA gene (encoding β-glucoronidase; GUS). In the future the aforementioned transformed Agrobacterium cells will be used to transform Arabidopsis plants to confirm the interactions of the above mentioned proteins with TSN and its association with SG and PB.

Main title:Towards the identification of Tudor SN interactome
Subtitle:cloning of putative interactors
Authors:Elander, Pernilla
Supervisor:Moschou, Panagiotis Nikolaou and Gutierrez, Emilio
Examiner:Sundström, Jens
Series:Examensarbete / Institutionen för växtbiologi, SLU
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2015
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:NK002 Biotechnology - Bachelor's Programme 180 HEC
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Keywords:Tudor staphylococcal nuclease, cloning, stress granules, processing bodies, programmed cell death
URN:NBN:urn:nbn:se:slu:epsilon-s-5265
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-5265
Subjects:Plant genetics and breeding
Plant physiology and biochemistry
Language:English
Additional Information:No ISSN for this series. The ISSN in the full-text is not valid.
Deposited On:25 Apr 2016 09:20
Metadata Last Modified:06 Feb 2017 10:14

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