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Hillerdal, Carl-Olof, 2009. Laminin motifs inserted into a recombinant spider drag-line silk protein increase the proliferation of human dermal fibroblasts. Second cycle, A2E. Uppsala: SLU, Dept. of Anatomy, Physiology and Biochemistry

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Abstract

Spider silk is a promising biomaterial. It is highly biocompatible, non-toxic, most likely naturally degraded by the host organism over time and strong and resilient. Importantly, it consists of proteins and new motifs can relatively easily be added. While native spider silk is not possible to produce in available systems, a shorter segment of the protein, 4repCt, coding for 4 repetive alanine blocks and the C-terminal, can be inserted in vector and expressed in E-coli. Three laminin-motifs were added to 4repCt; RGD, IKVAV and YIGSR, in order to test how this affects cell proliferation of dermal fibroblasts. The results, although not confirmed with statistical certainty, strongly suggests that dermal fibroblast proliferation increase when grown on fibers with said motifs inserted compared to unmodified fiber.

Fibers made with 4repCt are not as strong as native spider silk fibers. Therefore, cysteine was inserted into 4repCt, which allows production of potentially stronger fibers that can be subjected to tensile strength tests and disulphide brigde formation tests.

Main title:Laminin motifs inserted into a recombinant spider drag-line silk protein increase the proliferation of human dermal fibroblasts
Authors:Hillerdal, Carl-Olof
Supervisor:Hedhammar, My
Examiner:Eriksson, Staffan
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2009
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:None
Department:(VH) > Dept. of Anatomy, Physiology and Biochemistry
Keywords:4repCt, Spider dragline silk, RGD, YISGR, IKVAV, extracellular matrix, fibroblasts, biomaterial, laminin
URN:NBN:urn:nbn:se:slu:epsilon-8-418
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-8-418
Subjects:Chemistry
Life sciences
Language:English
Deposited On:04 Feb 2010 13:47
Metadata Last Modified:20 Apr 2012 14:11

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