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Sjöberg, Patrick, 2006. Detection of tobacco rattle tobravirus and potato mop-top pomovirus in Swedish soil samples by the use of different bait plants and analysis by ELISA and biotest.. First cycle, G2E. Alnarp: SLU, Department of Plant Protection Biology

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Abstract

Tobacco rattle tobravirus (TRV) and potato mop-top pomovirus (PMTV) are two known
viruses in Sweden which impair the quality of potato tubers by causing spraing. This
experiment aimed at finding the best bait plants (plants that is susceptible to infection)
for acquiring virus particles from soil, and to analyse the acquired viruses by both ELISA
and biotest. The results were finished after 8 weeks with findings that indicate differences
in the ability of acquiring virus particles between the bait plants. Nicotiana clevelandii and
N.debneyi seem to be the best bait plants to use. These plants were able to acquire both plant
viruses in high concentrations. ELISA results also verify that the vector for PMTV,
Spongospora subterranea f.sp. subterranea, and the vector for TRV,
Trichodorus/Paratrichodorus were present in the soil.
Another interesting discovery was that the only positive result of PMTV was found in soil
samples that had been dried for at least two weeks. This could be confirmed by later tests that
were made on a new batch of soil samples.
Five different indicator plants (plants that develop symptoms after inoculated with virus) were
used for the biotest and out of these Chenopodium amaranticolor and C.quinoa seem to be the
best to use because of the clearly visible local lesions that were easy to count. The biotest
results were compared to the ELISA results and the plants that showed local lesions matched
with positive ELISA values but the amount of lesions could not be correlated to high or low
ELISA values. A combination of these techniques could be used in future work to increase the
certainty of the diagnosis.

Main title:Detection of tobacco rattle tobravirus and potato mop-top pomovirus in Swedish soil samples by the use of different bait plants and analysis by ELISA and biotest.
Authors:Sjöberg, Patrick
Supervisor:Åhman, Gunilla
Examiner:Merker, Arnulf
Series:Bachelor project in the Danish-Swedish Horticulture programme
Volume/Sequential designation:2006:12
Year of Publication:2006
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:YHORT Horticultural Science Programme (admitted after 2000 and before July 1, 2007) 300 HEC
Department:(LTJ, LTV) > Department of Plant Protection Biology
Keywords:tobacco rattle tobravirus, potato mop-top pomovirus, ELISA, biotest, bait plants
URN:NBN:urn:nbn:se:slu:epsilon-4-150
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-4-150
Subjects:Plant diseases
Language:English
Deposited On:07 Dec 2009 08:20
Metadata Last Modified:22 Mar 2015 15:14

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