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Lidemalm, Louise, 2013. Characterisation of Wheat dwarf virus isolates from the vector Psammotettix alienus. First cycle, G2E. Uppsala: SLU, Department of Plant Biology (from 140101)

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Abstract

Wheat dwarf disease caused by Wheat dwarf virus (WDV) can cause a lot of damage in wheat fields by reduction of yield. WDV belongs to the genus Mastrevirus in the family of
Geminiviridae and it is transmitted by the leafhopper Psammotettix alienus. Viruses in the genus Mastrevirus have a genome of single-stranded (ss) circular DNA ranging in size from 2.6 kb – 2.8 kb. Mastreviruses have monopartite genomes with three genes: a movement protein (MP) gene, coat protein (CP) gene, and Rep. Replication-associated protein (Rep) and replication-associated protein A (RepA) are both translated from Rep, but from two different
mRNAs. For Rep, an intron has been spliced out, while for RepA the intron has been kept. The aim of this study was to see if the WDV isolates from samples collected in Skåne,
Sweden during 2010 was a new genotype, by analysing the DNA sequences. The samples from Skåne were of interest because for many years there have been no records of wheat
dwarf disease in Skåne. The hypothesis was that there was no new genotype of WDV and thus, that no recombination have occurred. To test the hypothesis DNA was extracted from
leafhoppers collected at different locations in Skåne and analysed with PCR, rolling circle amplification and digestion with restriction enzymes. To be able to use the total DNA for analysis a new method for extracting the DNA from leafhoppers was tested, which was verified to be successful. Alignments and a phylogenetic analysis were performed in MEGA 5.1 of the sequences obtained from the analysed samples, sequences from GenBank, and the master thesis by Yazdkhasti (2012). From the sequence analysis it could be confirmed that WDV from the wheat strain WDV-E was present in the samples with a high nucleotide identity so the conclusion was drawn that the isolates are close relatives to isolates from Sweden, China, and the rest of Europe. From the sequence and phylogenetic analyses it could
be seen that it was likely that there had been no major recombination in the genome and that it was no new genotype of WDV.

Main title:Characterisation of Wheat dwarf virus isolates from the vector Psammotettix alienus
Authors:Lidemalm, Louise
Supervisor:Kvarnheden, Anders
Examiner:Sohlberg, Joel
Series:Examensarbete / Institutionen för växtbiologi och skogsgenetik, SLU
Volume/Sequential designation:138
Year of Publication:2013
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:NK002 Biotechnology - Bachelor's Programme 180 HEC
Department:(NL, NJ) > Department of Plant Biology (from 140101)
Keywords:diversity, leafhopper, PCR, phylogenetic analysis, Psammotettix alienus, rolling circle amplification, sequence, vector, wheat, wheat dwarf disease, Wheat dwarf vector, whole genome amplification
URN:NBN:urn:nbn:se:slu:epsilon-s-3052
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-3052
Subjects:Pests of plants
Plant diseases
Language:English
Deposited On:25 Feb 2014 14:55
Metadata Last Modified:27 Feb 2014 07:40

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