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Pettersson, Martin, 2013. Stump treatment with the root rot antagonist phlebiopsis gigantea : - sensitivity of P. gigantea spores to high pressure stress; - reduced water consumption for stump treatment. Second cycle, A2E. Alnarp: SLU, Southern Swedish Forest Research Centre

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Abstract

Stump treatment today requires large quantities of clean water mixed with the protective product Rotstop, to fully cover the stump surface. In order to reduce the
water consumption without reducing the coverage of the stump surface, the pressure in the stump treatment device can be increased. Therefore, the Phlebiopsis gigantea
(Fr.) Jül. suspension used as a biological control agent to prevent Heterobasidion annosum (Fr.) Bref. infection was pressure-tested. The pressurized suspensions were
compared with the control suspensions and the survival was monitored by measuring oidia germination on agar medium. Pressures up to 150 bars (15 000 kPa) were tested
and showed no negative effects on the survival of the P. gigantea spores.

Three Phlebiopsis gigantea suspensions, with the same quantity of P. gigantea spores but with different water amounts (the recommended water amount 1 l/m2
stump surface, a reduced amount of 0.5 l/m2 and an even more reduced amount of 0.25 l/m2), were compared with each other. This was done to investigate if it is possible to reduce the water consumption for stump treatment and thereby reduce stump treatment costs. The experiment was conducted on stem pieces (billets) of Norway spruce (Picea abies (L.) Karst.) in a laboratory, and the amount of
Heterobasidion infection was screened. Results showed no difference between using 1, 0.5 or 0.25 l/m2.

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Vid stubbehandling går det åt en stor mängd vatten. Om trycket i apparaturen ökas kan vattenförbrukningen minskas utan att täckningsgraden av stubbenytan försämras.
I denna studie trycktestades Phlebiopsis gigantea (Fr.) som används vid biologisk stubbehandling för att förhindra angrepp av rotrötesvampen Heterobasidion annosum (Fr.) Bref. De trycksatta P. gigantea sporerna jämfördes mot en kontroll utan tryck.

Groningsförsök på agar-plattor visade att tryck upp till 150 bar (15 000 kPa) inte påverkade sporerna. Tre olika behandlingar jämfördes med varandra för att undersöka om det var möjligt att reducera vattenåtgången vid stubbehandling. En vanlig behandling med 1 l/m2 stubbyta, en behandling med 0.5 l/m2 och en behandling med 0.25 l/m2 jämfördes med varandra. Mängden P. gigantea sporer var exakt den samma för de olika behandlingarna. Experimentet utfördes på stambitar av Gran (Picea abies (L.) Karst.) i
ett laboratorium och mängden Heterobasidion-infektion mättes. Resultatet visade ingen skillnad mellan att använda 1, 0.5 eller 0.25 l/m2.

Main title:Stump treatment with the root rot antagonist phlebiopsis gigantea
Subtitle:- sensitivity of P. gigantea spores to high pressure stress; - reduced water consumption for stump treatment
Authors:Pettersson, Martin
Supervisor:Rönnberg, Jonas and Oliva, Jonàs
Examiner:Agestam, Eric
Series:Examensarbete / SLU, Institutionen för sydsvensk skogsvetenskap
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2013
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:SY001 Forest Science - Master's Programme 300 HEC
Department:(S) > Southern Swedish Forest Research Centre
Keywords:Stump treatment, Heterobasidion annosum, Heterobasidion parviporum, Phlebiopsis gigantea, Picea abies, Rotstop, pressure stress, water consumption
URN:NBN:urn:nbn:se:slu:epsilon-s-2656
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-2656
Subjects:Forestry - General aspects
Language:English
Deposited On:23 Aug 2013 14:41
Metadata Last Modified:23 Aug 2013 14:41

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