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Farragher, Naomi, 2013. Degradation of pesticides by the ligninolytic enzyme Laccase : optimisation of in vitro conditions, immobilisation and screening for natural mediators. Second cycle, A2E. Uppsala: SLU, Dept. of Microbiology



Pesticides are widely used in many industries but the majority reaches non-target organisms or locations through point or diffuse sources. Understanding conditions for their degradation is therefore important. The degradation of glyphosate, its metabolite AMPA and isoproturon using the ligninolytic enzyme laccase was studied. Optimisation of in vitro conditions were tested with findings indicating that factors such as altering pH and the concentrations of both manganese and redox mediators can impact degradation giving insight into optimal conditions. A method of encapsulation was used showing it is possible to immobilise laccase suggesting a possibility of its suitability as a co-formulation agent in pesticide applications. The immobilised laccase was applied in a laboratory scale experiment to investigate degradation of glyphosate and AMPA in soil and sand. The findings showed an apparent ability of the encapsulated laccase to be liberated and have an effect on glyphosate degradation, although much work still remains in this area. In the final part of this project ligninolytic substrates were screened for natural and easily extractable mediators. Extracts were used to check enzymatic activity and degradation potential. Candidates that showed promising results included extracts from hemp and wheat.

Main title:Degradation of pesticides by the ligninolytic enzyme Laccase
Subtitle:optimisation of in vitro conditions, immobilisation and screening for natural mediators
Authors:Farragher, Naomi
Supervisor:Pizzul, Leticia
Examiner:Stenström, John
Series:Examensarbete / Sveriges lantbruksuniversitet, Institutionen för mikrobiologi
Volume/Sequential designation:2013:6
Year of Publication:2013
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM001 Environmental Pollution and Risk Assesment - Master's Programme 120 HEC
Supervising department:(NL, NJ) > Dept. of Microbiology
Keywords:White rot fungi, Laccase, Bioprophylaxis, Diffuse sources, Co-formulation, Degradation, Mediators, Pesticides, Glyphosate, AMPA, Isoproturon, Enzyme encapsulation, Enzyme immobilisation, Natural mediators
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Subject. Use of subject categories until 2023-04-30.:Pests of plants
Soil science and management
Deposited On:18 Jun 2013 13:35
Metadata Last Modified:18 Jun 2013 13:41

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