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Khajouei, Ehsan, 2013. Molecular characterization of the faecal fungal flora in healthy horses. Second cycle, A2E. Uppsala: SLU, Dept. of Animal Nutrition and Management (until 231231)



There is a lack of knowledge on the composition and dynamics of the intestinal micro-eukaryotes of horse and to date their functional role for the health and
physiology of the host has been described merely to some extent. Particularly, no culture-independent surveys of the dynamics of the intestinal fungal flora in horse are
available. Two culture-independent approaches, terminal restriction fragment length polymorphism (T-RFLP) and cloning & sequencing, were combined to study the
composition and dynamics of the intestinal fungal flora in healthy horses. The fungal internal transcribed regions (ITS) and the eukaryotic 18S rRNA genes, recovered
from faecal samples of 16 Swedish Standardbred geldings, were used as phylogenetic marker genes in the analyses. Faecal samples were analyzed by TRFLP and two clone libraries were constructed, one library for the 18S gene and the other for the ITS gene from which later 44 random clones were sequenced. T-RFLP profiles and sequencing data together, indicate that the fungal community is temporally stable, has a low diversity and it is dominated by three genera of the Neocallimastigaceae family including Orpinomyces sp., Anaeromyces sp. and Piromyces species. These fungal species have been isolated and characterized
previously in different types of herbivores including the Equidae family. These anaerobic fungi have been suggested to exist prevalently in the GI tract of large
herbivores which in this aspect, there is no disparity between the data presented in this experiment and those from previous publications. T-RFLP, as a high-throughput
culture-independent method which so far has been implemented mainly on the studies targeting the prokaryotic members of the microbiota, is suitable for the survey
of the faecal fungal flora of horses, however requires further adaptations.

Main title:Molecular characterization of the faecal fungal flora in healthy horses
Authors:Khajouei, Ehsan
Supervisor:Dicksved, Johan
Examiner:Lindberg, Jan Erik
Series:Examensarbete / Sveriges lantbruksuniversitet, Institutionen för husdjurens utfodring och vård
Volume/Sequential designation:421
Year of Publication:2013
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:VM003 Animal Science - Master's Programme 120 HEC
Supervising department:(VH) > Dept. of Animal Nutrition and Management (until 231231)
Keywords:Anaerobic fungi, Equine, Eukaryotic microorganism, Fungal ITS gene, Gut microbiota, Horse, 18S rRNA gene, Neocallimastigaceae family
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Subject. Use of subject categories until 2023-04-30.:Animal feeding
Animal physiology - Nutrition
Life sciences
Deposited On:13 Jun 2013 10:52
Metadata Last Modified:13 Jun 2013 10:52

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