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Matzén, Staffan, 2012. Effect of Bacillus mediated priming on different natural genetic variants of Arabidopsis thaliana. First cycle, G2E. Uppsala: SLU, Dept. of Plant Biology and Forest Genetics (until 131231)

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Abstract

In two feeding experiments Bacillus induced priming of plant defense was tested for its effect against the generalist insect pest Spodoptera littoralis. The main hypothesis for the study was that Bacillus induced priming would enable the plant Arabidopsis thaliana to defend itself
better against this insect herbivore since this priming seems to involve jasmonic acid, known to be important for plant defense to insects. A secondary objective of the study was to examine if any differences in a primed defense capability could be seen between different natural genetic variants (ecotypes) of A. thaliana reflecting habitat differences in pest pressure.

The methods used for plant cultivation and Bacillus inoculation were aimed at reflecting conditions that can be expected in an agricultural cropping system. This meant that all plants were cultivated in soil, and the feeding experiments were conducted with the plants growing intact in the soil system. Inoculation of plants was done with the Bacillus bacteria in its stress resistant spore form. A literature survey was performed to gather knowledge involving some of the more important concepts in plant defense against herbivores. The glucosinolate/myrosinase system as well as defense activation was studied in more detail.

The study concluded that although Bacillus mediated priming was found to have an effect on some induced defenses in A. thaliana, no enhanced resistance against an insect herbivore was observed within the experiments conducted.

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I två olika matningsförsök testades om växters försvarsförmåga mot den herbivora insekten
Spodoptera littoralis påverkades av priming med Bacillus bakterier. Hypotesen för studien var att Bacillus inducerad priming skulle stärka försvaret mot herbivora insekter hos
Arabidopsis thaliana. Hypotesen grundade sig på information som indikerade att denna priming involverade Jasmonsyra systemet som visat sig vara en viktig del i växters försvar mot insekter. Ett sekundärt mål för studien var att undersöka eventuella skillnader i Bacillus primad försvarsförmåga mellan olika ekotyper av A. thaliana som skulle kunna bero på skillnader i skadegörartryck mellan olika habitat.

Metoderna som användes vid odling av växter och inokulering med Bacillus syftade till att likna förhållanden som kan förväntas i agrara odlingssystem. Detta innebar att alla växter som användes i försöken odlades i jord från groning till avslutat experiment samt att inokuleringen gjordes med Bacillus i sin tåliga sporform. En litteraturstudie gjordes för att samla kunskap om de viktigare koncepten i växtförsvar mot herbivora insekter. Glukosinolat/myrosinas
systemet samt aktiveringen av försvarssystem studerades mer ingående.

Studien kunde inte påvisa någon ökad motståndskraft mot en herbivor insekt till följd av Bacillus priming. Detta trots att denna form av priming konstaterades ha en effekt på några delar av det inducerbara försvaret i A. thaliana.

Main title:Effect of Bacillus mediated priming on different natural genetic variants of Arabidopsis thaliana
Authors:Matzén, Staffan
Supervisor:Meijer, Johan and Bejai, Sarosh
Examiner:Kvarnheden, Anders
Series:Examensarbete / Institutionen för växtbiologi och skogsgenetik, SLU
Volume/Sequential designation:Nr: 129
Year of Publication:2012
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:NY003 Agricultural Programme - Soil/Plant 270 HEC
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Keywords:priming of defense, generalist herbivore, Spodoptera littoralis, Arabidopsis thaliana, glucosinolate, feeding experiment, pest management
URN:NBN:urn:nbn:se:slu:epsilon-s-1593
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-1593
Subjects:Agricultural research
Plant ecology
Pests of plants
Language:English
Deposited On:30 Aug 2012 14:44
Metadata Last Modified:30 Aug 2012 14:44

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