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Malmrot , Astrid , 2010. Begomovirus prevalence and diversity in the TYLCV resistant tomato cultivar Shanty in Nicaragua . First cycle, G2E. Uppsala : SLU, Dept. of Plant Biology and Forest Genetics (until 131231)

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Abstract

Every year begomovirus infections cause severe constraints to the tomato production in Nicaragua. Extensive yield losses are followed by large economical losses, both for the country and the single farmer. During recent years the use of begomovirus resistant or tolerant tomato cultivars has increased in Nicaragua as an effort to improve yields. Still, begomovirus infection is a large threat to the tomato production. Therefore, ongoing work in Nicaragua is focusing on finding new, better, begomovirus tolerant or resistant tomato cultivars. This study aims to be a part of this work, by investigating the current prevalence and diversity of begomoviruses in the resistant tomato variety Shanty. Nicaraguan tomato plants of the variety Shanty were collected from four geographical locations around Nicaragua (Sebaco, CNIAB (close to Managua), Las Playitas, Tecolostote). The collected samples were investigated for begomovirus infection using polymerase chain reaction (PCR) and universal begomovirus primers. Infection was confirmed at all locations where plants with clear symptoms of infection had been collected. Infection was also detected in plants, which had been collected without any visible symptoms of begomovirus infections. Rolling circle amplification (RCA) produced a suitable product for restriction analysis in six of the begomovirus positive samples. Restriction analysis with the three restriction enzymes EcoRI, SalI and HpaII, showed mixed infection (more than one begomovirus species present in the tomato plant) in ~80% of the analysed samples. The results showed that the most common tomato producing areas in Nicaragua (Sebaco, CNIAB, Las Playitas, Tecolostote) still are heavily exposed to begomovirus infections and that mixed viral infections are relatively common in these regions.

Main title:Begomovirus prevalence and diversity in the TYLCV resistant tomato cultivar Shanty in Nicaragua
Authors:Malmrot , Astrid
Supervisor:Kvarnheden , Anders
Examiner:Westerbergh, Anna
Series:Independent project / Department of Plant Biology & Forest Genetics, SLU
Volume/Sequential designation:114
Year of Publication:2010
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:NK002 Biotechnology - Bachelor's Programme 180 HEC
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Keywords:begomovirus , mixed viral infections , resistance , restriction analysis , tomato cultivar , Nicaragua
URN:NBN:urn:nbn:se:slu:epsilon-s-5475
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-5475
Subjects:Agricultural research
Plant genetics and breeding
Plant diseases
Language:English
Deposited On:25 Oct 2010 08:03
Metadata Last Modified:20 Jun 2016 14:50

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