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Lukic, Robin, 2023. Cultivating oleaginous yeast on spent mushroom substrate and logging residues hydrolysate : an assessment of yeast growth on side product substrates from the food, agriculture, and forestry industry. Second cycle, A2E. Uppsala: SLU, Department of Molecular Sciences

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Abstract

There is an interest in finding alternatives to vegetable oils because of negative environmental impact. Many green sectors generate an abundant amount of side products that are inedible and consist in large of lignocellulose rich materials. Oleaginous yeast can ferment inedible substrates and accumulate a high amount of lipids that have a similar fatty acid profile to that of vegetable oils. Various screening methods exist to assess microbial growth with the aid of colour changing redox indicators. Two yeast strains, Rhodotorula toruloides CBS14 and Rhodotorula babjevae DVPG8058, were screened in a microtiter plate (MTP) system (Applikon Micro-Flask) with a tetrazolium salt as indicator (TTC). The strains were also cultivated on spent mushroom substrate hydrolysate (SMSH) and logging residues hydrolysate (LRH) as substrates. The TTC indicator likely caused inhibition at 1.0 g/l. It was visible at lower concentrations (0.1 and 0.05 g/l) but permanently stained the MTPs. The SMSH had the most positive impact on yeast growth while the cellulose fraction (CF) and hemicellulose fraction (HF) of the LRH showed less overall growth and inhibitory effects. The HF caused high inhibition in both yeast strains, but they could grow at 30% dilution. The CF had similar concentrations of glucose, xylose, and acetic acid as the SMSH but showed less growth. The SMSH could need less pre-treatment than LRH and possibly not need to be supplemented with nutrients because of mushroom residues. The Applikon-Micro Flask system proved sufficient for screening of yeasts on several substrates.

Main title:Cultivating oleaginous yeast on spent mushroom substrate and logging residues hydrolysate
Subtitle:an assessment of yeast growth on side product substrates from the food, agriculture, and forestry industry
Authors:Lukic, Robin
Supervisor:Müller, Bettina and Blomqvist, Johanna
Examiner:Passoth, Volkmar
Series:Molecular Sciences
Volume/Sequential designation:2023:13
Year of Publication:2023
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NY010 Agronomprogrammet - livsmedel, för antagna fr.o.m. 2016 300 HEC
Supervising department:(NL, NJ) > Department of Molecular Sciences
Keywords:Lignocellulose, side products, oleaginous yeast, yeast oil, microtiter plate system, redox indicator, yeast growth, spent mushroom substrate hydrolysate, logging residues hydrolysate
URN:NBN:urn:nbn:se:slu:epsilon-s-19053
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-19053
Language:English
Deposited On:07 Jul 2023 07:58
Metadata Last Modified:08 Jul 2023 01:02

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