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Sjöberg, Caroline, 2023. Anaerobic cellulose degrading bacteria isolated from biogas plants. Second cycle, A2E. Uppsala: SLU, Department of Molecular Sciences



Two cellulose degrading bacteria, named BCISO-4 and DC1-18-4, were isolated from samples retrieved from two large scale biogas-plants. In order to investigate and determine information regarding their growth preferences multiple tests were performed.
Growth for the two isolates was tested on the carbon sources: cellobiose, starch, hemicellulose, straw, cellulose, fructose, glucose, ribose, mannose, xylose and sorbitol (0.5w%/v.). Strong growth was documented on cellulose and cellobiose for both organisms and for BCISO-4 weak growth was possible with xylose, starch, sorbitol, glucose, fructose and ribose. For DC1-18-4 weak growth was observed with xylose, straw, glucose and ribose. Growth was also tested on a more complex nutrient medium, reinforced clostridial medium (RCM), no growth was however documented.
The major products produced during growth, as analyzed by HPLC, were determined to be acetate and butyrate for both organisms. For BCISO-4 and DC1-18-4 the growth span for temperature and pH was determined to 25-54 ° C and pH 6-10, and 25-51 ° C and pH 6-9, respectively. Enzyme activity for free enzymes as well as cell-bound was tested on glucose and xylose, and all tests indicated activity.
Terminal restriction fragment length polymorphism analysis (TRFLP) targeting the cel 5 gene was performed on samples from the biogas-plants to investigate presence of the isolated species, which confirmed their presence.
Keywords: anaerobic bacteria, biogas, cellulose, cellobiose, VFA, enzyme activity, TRFLP

Main title:Anaerobic cellulose degrading bacteria isolated from biogas plants
Authors:Sjöberg, Caroline
Supervisor:Schnürer, Anna
Examiner:Jonsson, Hans
Series:Molecular Sciences
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2023
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:Other
Supervising department:(NL, NJ) > Department of Molecular Sciences
Keywords:anaerobic bacteria, biogas, cellulose, cellobiose, VFA, enzyme activity, TRFLP
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Subject. Use of subject categories until 2023-04-30.:Life sciences
Deposited On:02 May 2023 07:12
Metadata Last Modified:03 May 2023 01:01

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