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Rydmark, Hanna, 2023. Replacement of fish protein in fish feed : effects on the yeast flora in the gut of Arctic charr (Salvelinus alpinus). Second cycle, A2E. Uppsala: SLU, Dept. of Animal Nutrition and Management

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Abstract

The aim with this study was to investigate how feeds with different protein sources influence the levels and composition of the yeast flora in the gut of Arctic charr (Salvelinus alpinus). Arctic charr were fed for two weeks on a control diet (C) with fishmeal (FM) or experimental diets with 40% FM replaced by yeast (Saccharomyces cerevisiae) (Y), meal from blue mussel (Mytilus edulis) (M) or a feed with Baltic Sea originated protein; 33% FM, 33% mussel meal (MM) and 33% S. cerevisiae referred to as Baltic blend (BB). The effect of the different feeds was evaluated based on fish growth and the effects on the microbial population, with a focus on yeasts, in different parts of the gut (stomach (S), pylorus (P), mid intestine (MI) and distal intestine (DI)). Differences in the development of the yeast flora composition and yeast loads were investigated using agar plate counts and yeast species identification using polymerase chain reaction (PCR) amplification combined with sequencing of the 28S rRNA gene. The amount of bacteria and moulds were also estimated when examining the agar plates. In addition, the different feeds were analysed for yeast load, yeast species composition and diversity. The study showed that there were differences in yeast load in the gut linked to diet, where the diet containing S. cerevisiae (Y) had a higher yeast load compared to the other diets both before (C: p = 0.003, M: p = 0.016, BB: p = 0.0007) and after the diet intervention (C: p = 0.024, M: p = 0.001, BB: p = 0.001). Differences in amount of yeast could also be linked to time (i.e., before and after) (p = 0.0009) with greater yeast loads at the end of the experiment, where the yeast load in the gut ranged between 4.1–7.5 log CFU g-1 after two weeks of dietary treatment. However, no differences between gut segments were found on yeast load or yeast composition. The domination yeast species found in gut in all diets were Debaryomyces hansenii (68–70% of yeast isolates) followed by Debaryomyces sp. (19–24%). Both D. hansenii and Debaryomyces sp. were more abundant at the end of the study. No differences in yeast composition were found between diets. In all feeds, D. hansenii was found and S. cerevisiae was detected in all feeds except in the control feed (C). The feed with 40% FM replaced by S. cerevisiae (Y) had the highest yeast load with D. hansenii as the dominating yeast specie. The different experimental diets did not impact weight or length between the fish in the study after two weeks trial. The study design lacked tank as a factor for statistical analysis. Hence, the results might only be viewed as indications. Further research is necessary for continued understanding of the feed impact on the yeast flora in gut of Arctic charr.

Main title:Replacement of fish protein in fish feed
Subtitle:effects on the yeast flora in the gut of Arctic charr (Salvelinus alpinus)
Authors:Rydmark, Hanna
Supervisor:Dicksved, Johan
Examiner:Lundh, Torbjörn
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2023
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:VY001 Agricultural Science Programme - Animal Science 270 HEC
Supervising department:(VH) > Dept. of Animal Nutrition and Management
Keywords:Microbiota, Saccharomyces cerevisiae, fishmeal, mussel meal, Mytilus edulis, aquaculture
URN:NBN:urn:nbn:se:slu:epsilon-s-18590
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-18590
Subject. Use of subject categories until 2023-04-30.:Animal feeding
Language:English
Deposited On:03 Feb 2023 08:01
Metadata Last Modified:04 Feb 2023 02:02

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