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Dölfors, Fredrik, 2018. Characterisation of the epistatic hybrid incompatibility locus STRUBBELIG RECEPTOR FAMILY 3 in Arabidopsis. Second cycle, A2E. Uppsala: SLU, Department of Plant Biology (from 140101)

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Abstract

Populations of Arabidopsis thaliana have spread throughout temperate zones of the world and adapted to prevailing biotic and abiotic stress factors. Diverging popula-tions of Arabidopsis accessions can evolve postzygotic hybrid incompatibility (HI). In some cases, HI is caused by incompatible epistatic interactions between genes that have functionally diverged between- and within species. Crosses between the Euro-pean Landsberg erecta (Ler) and many central Asian accessions, like Kashmir-2 (Kas-2) and Kondara (Kond) suffers from it. Genetic analysis of this incompatibility has revealed its basis in a polymorphic cluster of Toll/interleukin-1 receptor-nucleo-tide binding-leucine rich repeat (TNL) RPP1 (Recognition of Peronospora parasitica 1)-like genes in Ler and alleles of the receptor-like kinase Strubbelig Receptor Fam-ily 3 (SRF3) in ecotypes Kas-2 and Kond, causing autoactivation of the plant innate immunity response. In this project I have analysed: I) the genetic variability of SRF3 in a segregating population of Ler relatives originally from Gorsów (Poland) and II) the molecular function of SRF3. My analysis showed that SRF3 might be more con-served in the Gorsow population compared to the Kas-2 and Kond accessions alt-hough it does not provide insight in the evolution of incompatibility. Analysis of the role of SRF3 using overexpressing and mutant lines and in silico prediction indicates that it might be involved in both drought and salt stress. In summary, this project sheds light into the role of SRF3 as a stress-related gene and its implication in hybrid incompatibility.

Main title:Characterisation of the epistatic hybrid incompatibility locus STRUBBELIG RECEPTOR FAMILY 3 in Arabidopsis
Authors:Dölfors, Fredrik
Supervisor:Martinez Arias, German
Examiner:Dixelius, Christina
Series:Examensarbete / Institutionen för växtbiologi, SLU
Volume/Sequential designation:169
Year of Publication:2018
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM008 Plant Biology - Master's Programme 120 HEC
Supervising department:(NL, NJ) > Department of Plant Biology (from 140101)
Keywords:abiotic stress, arabidopsis, co-evolution, hybrid incompatibility, receptor-like kinase
URN:NBN:urn:nbn:se:slu:epsilon-s-10185
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-10185
Subjects:Plant physiology - Reproduction
Language:English
Additional Information:Collaborator: Rubén Alcázar, Department of Biology, Healthcare and Environment. University of Barcelona. Faculty of Pharmacy. Avda Joan XXIII 27-31, 08028 Barcelona, Spain
Deposited On:01 Feb 2019 12:18
Metadata Last Modified:02 Feb 2019 02:00

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