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Ahmad, Tauseef, 2015. Amino acid deprivation of the hypothalamic cell-line N25/2 induces changes in regulation of numerous transporters from the SLC family. Second cycle, A2E. Uppsala: SLU, Dept. of Microbiology



Amino acids are the building blocks of proteins. They are essential for cell survival and normal cellular functions as they are the key precursors to numerous cellular pathways and processes. Availability of amino acids exhibit vital role in regulation of gene expression in all mammalian cells. Transporter proteins mediate transport of amino acids across the plasma membrane. In this study, complete amino acid starvation was performed on the hypothalamic cell line 25/2 from mouse to investigate the regulation of gene expression of amino acid transporters. We monitored up and down regulation in transcription of genes encoding solute carriers (Slcs) with a primary focus on amino acid transporters. Amino acid transporters from system A, L, xc, y+ and system ASC showed a response to amino acid starvation. Members from various solute carrier families that transport amino acids as their preferred substrate were found to be upregulated after 3-5 hours. Moreover, a few orphan genes such as Slc23a3 and Mfsd11 with unknown substrate profile were also found to be upregulated in response to amino acid depletion.

Main title:Amino acid deprivation of the hypothalamic cell-line N25/2 induces changes in regulation of numerous transporters from the SLC family
Authors:Ahmad, Tauseef
Supervisor:Blomqvist, Johanna and Fredriksson, Robert
Examiner:Passoth, Volkmar
Series:Examensarbete / Sveriges lantbruksuniversitet, Institutionen för mikrobiologi
Volume/Sequential designation:2015:5
Year of Publication:2015
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM003 Biotechnology - Master's Programme 120 HEC
Supervising department:(NL, NJ) > Dept. of Microbiology
Keywords:solute carriers, amino acid starvation, mTOR Pathway, AAR Pathway, amino acid transport systems, membrane transport proteins, amino acid transporters
Permanent URL:
Subject. Use of subject categories until 2023-04-30.:Animal genetics and breeding
Life sciences
Deposited On:02 Sep 2015 15:20
Metadata Last Modified:02 Sep 2015 15:20

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