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Jeppsson, Simon, 2015. Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica. Second cycle, A2E. Alnarp: SLU, Department of Plant Breeding (from 130101)

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Abstract

BACKGROUND: Crambe is an oil-crop suitable for industrial purposes and breeding aiming at producing novel oil-qualities may be achieved by genetransformation.
Understanding the synthesis of fatty acids and an efficient
transformation protocol is of pivotal role in this context.
Two transformation protocols (Agrobacterium mediated according Bang-Quang Huang and oral dip according Clough and Bent 1998) for crambe are evaluated, and a RNAi construct is produced aiming to down-regulate CaDGAT1.
RESULTS: None of the evaluated transformation protocols produce any successfully transformed plants during this evaluation. CaDGAT1 is partially sequenced and a RNAi construct for CaDGAT is produced, while the functionality
of the construct is un-validated.
CONCLUSIONS: Alternative Agrobacterium mediated transformation protocols published by Li et al. 2010 have managed to transform crambe successfully but a functional
oral dip protocol may be even more cost effective.
more studies on oral dip must be carried out to evaluate this however.

Main title:Evaluation of two transformation protocols for Crambe hispanica subsp. abyssinica
Authors:Jeppsson, Simon
Supervisor:Carlsson, Anders
Examiner:Lager, Ida
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2015
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:YHORT Horticultural Science Programme (admitted after 2000 and before July 1, 2007) 300 HEC
Department:(LTJ, LTV) > Department of Plant Breeding (from 130101)
Keywords:Crambe, transformation, CaDGAT1, floral dip, industrial oil crop, tailored oil quality
URN:NBN:urn:nbn:se:slu:epsilon-s-4250
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-4250
Subjects:Life sciences
Language:English
Additional Information:Ämne: biologi
Deposited On:07 Apr 2015 09:09
Metadata Last Modified:07 Apr 2015 09:09

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