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Balagunaseelan, Navisraj, 2014. Evaluation of the gene expression of STING, IFN‐β and osteopontin in tissue obtained from pigs treated with Matrix‐M. Second cycle, A2E. Uppsala: SLU, Dept. of Biomedical Sciences and Veterinary Public Health

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Abstract

Matrix‐M is the adjuvant component in traditional ISCOM vaccines. The adjuvant when used along with antigen increases the efficiency of the vaccines by inducing a balanced Th1/Th2 response and a long‐lasting antibody response. Transcriptomal studies in the pig show that the majority of upregulated genes during the early inflammatory response to Matrix‐M alone are Interferon regulated genes (Ahlberg, Lovgren Bengtsson, Wallgren, & Fossum, 2012). The present study aimed to establish qPCR assays for detection of gene expression of stimulator of interferon genes (STING), osteopontin (OPN) and interferon‐beta (IFN‐β) in porcine tissue. STING is a protein localized in the
endoplasmatic reticulum that on activation is believed to enhance the production of Type I interferons (Sun et al., 2009). Overexpression of OPN is believed to play a key role in inducing the production of Interferon‐α and other Type 1 interferons by plasmacytoid dendritic cells (Shinohara
et al., 2006). A comparative gene expression study of STING and OPN in pigs treated with Matrix‐M revealed that one or both of the genes were up‐regulated in pigs with increased expression of the IFN‐β gene 24 hours after administration of Matrix‐M.

Main title:Evaluation of the gene expression of STING, IFN‐β and osteopontin in tissue obtained from pigs treated with Matrix‐M
Authors:Balagunaseelan, Navisraj
Supervisor:Hjertner, Bernt
Examiner:Fossum, Caroline
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2014
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:VY002 Veterinary Medicine Programme 330 HEC
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Keywords:STING , OPN, MATRIX‐M, type I interferons
URN:NBN:urn:nbn:se:slu:epsilon-s-4082
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-4082
Subjects:Animal diseases
Language:English
Deposited On:06 Feb 2015 11:39
Metadata Last Modified:06 Feb 2015 11:39

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