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Wang, Xiaoyu, 2009. Identification of proteins expressed by the root-rot fungus Heterobasidion annosum when growing on lignocellulose. Second cycle, A2E. Uppsala: SLU, Dept. of Molecular Biology (until 131231)

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Abstract

The Root-rot fungus, Heterobasidion annosum, is a major pathogen on spruce and pine trees and is estimated to cause up to 20 % production loss in the forest industry in the temperate region. The genome of H. annosum has recently been sequenced and a total of ~280 carbohydrate-active enzymes have been predicted so far. The fungus is apparently an efficient wood degrader and may produce enzymes of interest for enzymatic saccharification of cellulose for biofuel production. The aim of this study was to see which major enzymes that are expressed and utilized. The strain that was sequenced, H. annosum TC-32-1, was grown in minimal medium with different lignocelluloses as carbon source. Extracellular proteins from one culture with spruce as carbon source were detected in electrophoresis after separation by cation and anion exchange chromatography. Proteins were identified by tryptic peptide mapping using MALDI-TOF mass spectrometry. We have identified 15 proteins so far which included 1 polysaccharide lyase, 10 glycoside hydrolases and 4 hypothetical proteins. Cellulase activity measurements were carried out using p-hydroxybenzoic acid hydrazide (PHBAH) as reagent for reducing sugar. One Cel7 enzyme was identified as the most expressed protein, and showed the highest cellulase activity.

Main title:Identification of proteins expressed by the root-rot fungus Heterobasidion annosum when growing on lignocellulose
Authors:Wang, Xiaoyu
Supervisor:Ståhlberg, Jerry and Svedberg, Jesper and Sandgren, Mats
Examiner:Knight, Stefan
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2009
Level and depth descriptor:Second cycle, A2E
Department:(NL, NJ) > Dept. of Molecular Biology (until 131231)
Keywords:Heterobasidion annosum, peptide mapping, lignocellulose, cellulase, cellobiohydrolase, wood degradation, white-rot fungus
URN:NBN:urn:nbn:se:slu:epsilon-8-268
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-8-268
Subjects:Pests of plants
Renewable energy resources
Processing of forest products
Life sciences
Language:English
Additional Information:Master thesis intended for the Master Program in Applied Biotechnology at Uppsala University
Deposited On:15 Oct 2009 08:38
Metadata Last Modified:20 Apr 2012 14:10

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