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Rupp, Haley, 2013. Effects of ethylene on secondary xylem formation in Arabidopsis thaliana. Second cycle, A2E. Umeå: SLU, Dept. of Forest Genetics and Plant Physiology



Ethylene has myriad roles as a plant hormone, ranging from senescence and defending against pathogen attacks to fruit ripening and interactions with other hormones. It has been shown to increase cambial activity in poplar, but the effect on wood formation in Arabidopsis hypocotyl has not previously been studied. The Auxin-Regulated Gene involved in Organ Size (ARGOS), which increases organ size by lengthening the time for cell division, was found to be upregulated by ethylene. We tested the effect of ethylene treatment at 10 and 100 µM ACC on three genotypes of Arabidopsis, Col0 (wild-type), an ARGOS deficient mutant (argos), and ein3-1, an ethylene insensitive mutant. ARGOS expression analysis with qPCR indicated that ACC does induce ARGOS and ARGOS-LIKE (ARL) in the hypocotyl. As seen in poplar, ethylene also decreases stem elongation.Histochemical staining, showed that ethylene changes the way secondary xylem lignifies, causing gaps in lignification around the outer edge of secondary xylem. Our results also implied that ethylene treatment changes the proportion of secondary to total xylem, resulting in less secondary, whereas in poplar, ethylene treatment caused an increase.

Main title:Effects of ethylene on secondary xylem formation in Arabidopsis thaliana
Authors:Rupp, Haley
Supervisor:Sundberg, Björn and Felten, Judith
Examiner:Ljung, Karin
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2013
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:SM002 Plant and Forest Biotechnology - Master's Programme 120 HEC
Supervising department:(S) > Dept. of Forest Genetics and Plant Physiology
Keywords:Arabidopsis thaliana, Ethylene, ACC, Secondary xylem, Cell wall chemistry, Lignin
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Subject. Use of subject categories until 2023-04-30.:Forestry production
Deposited On:02 May 2013 08:50
Metadata Last Modified:22 Jan 2019 10:59

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