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Bergdahl, Roland, 2013. Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1. Second cycle, A2E. Uppsala: SLU, Dept. of Molecular Biology (until 131231)

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Abstract

The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolution, respectively. The FTHFS 1 crystal structure has four non-crystallographic related protein molecules in the asymmetric unit of the crystal, while FTHFS 2 has two. The pH optimum in the formylation reaction for FTHFS 1 was determined to be 7.5, while FTHFS 2 had a pH optimum of 9, and it was determined that both enzymes had a temperature optimum of 60°C for their formulation reactions. An attempt to perform the deformylation reaction with both enzymes was unsuccessfully carried out.

Main title:Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1
Authors:Bergdahl, Roland
Supervisor:Mikkelsen, NilsEgil
Examiner:Sandgren, Mats
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2013
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:None
Department:(NL, NJ) > Dept. of Molecular Biology (until 131231)
Keywords:Biogas, Syntrophic acetate oxidizing bacteria, Tepidanaerobacter acetatoxydans Re1, FTHFS, N10- formyltetrahydrofolate synthetase, Crystallography, Structure determination
URN:NBN:urn:nbn:se:slu:epsilon-s-1874
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-1874
Subjects:Renewable energy resources
Language:English
Additional Information:An embargo period of 6 months has been admitted for this MSc thesis work
Deposited On:04 Dec 2012 12:23
Metadata Last Modified:16 Jul 2013 23:15

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