Bergdahl, Roland, 2013. Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1. Second cycle, A2E. Uppsala: SLU, Dept. of Molecular Biology (until 131231)
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Abstract
The crystal structure of N10- formyltetrahydrofolate synthetase 1 (FTHFS 1) and N10- formyltetrahydrofolate synthetase 2 (FTHFS 2) from the syntrophic acetate oxidizing bacterium, Tepidanaerobacter acetatoxydans Re1, was solved by x-ray crystallography to a resolution of 2.15 Å, and 2.30 Å resolution, respectively. The FTHFS 1 crystal structure has four non-crystallographic related protein molecules in the asymmetric unit of the crystal, while FTHFS 2 has two. The pH optimum in the formylation reaction for FTHFS 1 was determined to be 7.5, while FTHFS 2 had a pH optimum of 9, and it was determined that both enzymes had a temperature optimum of 60°C for their formulation reactions. An attempt to perform the deformylation reaction with both enzymes was unsuccessfully carried out.
| Main title: | Characterization, crystallization and two three dimensional structures of the N10- formyltetrahydrofolate synthetase (FTHFS) from the syntrophic acetate oxidizing bacterium Tepidanaerobacter acetatoxydans Re1 |
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| Authors: | Bergdahl, Roland |
| Supervisor: | Mikkelsen, NilsEgil |
| Examiner: | Sandgren, Mats |
| Series: | UNSPECIFIED |
| Volume/Sequential designation: | UNSPECIFIED |
| Year of Publication: | 2013 |
| Level and depth descriptor: | Second cycle, A2E |
| Student's programme affiliation: | None |
| Supervising department: | (NL, NJ) > Dept. of Molecular Biology (until 131231) |
| Keywords: | Biogas, Syntrophic acetate oxidizing bacteria, Tepidanaerobacter acetatoxydans Re1, FTHFS, N10- formyltetrahydrofolate synthetase, Crystallography, Structure determination |
| URN:NBN: | urn:nbn:se:slu:epsilon-s-1874 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-1874 |
| Subject. Use of subject categories until 2023-04-30.: | Renewable energy resources |
| Language: | English |
| Additional Information: | An embargo period of 6 months has been admitted for this MSc thesis work |
| Deposited On: | 04 Dec 2012 12:23 |
| Metadata Last Modified: | 16 Jul 2013 23:15 |
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