Zhou, Mi, 2012. Identification of protein-interaction partners of ZBED6. Second cycle, A2E. Uppsala: SLU, Dept. of Animal Breeding and Genetics
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Abstract
ZBED6 was recently discovered as a novel transcriptional repressor of IGF2 because a mutation disrupting one of its binding sites in porcine IGF2 intron 3 leads to greater postnatal IGF2 expression in skeletal muscle and makes pig grow more muscle. ZBED6 is unique to placental mammals and derived from domesticated DNA transposon. However, the mechanism of ZBED6-mediated transcriptional regulation is still unknown. The study of the protein interacting partners of ZBED6 is a straightforward way to help us answer the question. By using the cells with stable overexpression of ZBED6, we perform one-step pull down assay and tandem affinity purification. A protein complex, SFPQ-Nono, is identified as the protein-interaction partners of ZBED6 by using mass spectrometry.
| Main title: | Identification of protein-interaction partners of ZBED6 |
|---|---|
| Authors: | Zhou, Mi |
| Supervisor: | Andersson, Göran and Andersson, Leif and Jiang, Lin |
| Examiner: | Ronne, Hans |
| Series: | Examensarbete / SLU, Institutionen för husdjursgenetik |
| Volume/Sequential designation: | 386 |
| Year of Publication: | 2012 |
| Level and depth descriptor: | Second cycle, A2E |
| Student's programme affiliation: | NM003 Biotechnology - Master's Programme 120 HEC |
| Supervising department: | (VH) > Dept. of Animal Breeding and Genetics |
| Keywords: | ZBED6, transcription factor, BAC TransgeneOmics, tandem affinity purification, protein interaction |
| URN:NBN: | urn:nbn:se:slu:epsilon-s-1871 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-1871 |
| Subject. Use of subject categories until 2023-04-30.: | Animal genetics and breeding |
| Language: | English |
| Deposited On: | 23 Nov 2012 15:05 |
| Metadata Last Modified: | 23 Nov 2012 15:12 |
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