Lundqvist, Urban, 2009. PLA som metod för detektion av bornavirusinfektion hos katt. Second cycle, A2E. Uppsala: SLU, Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
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Abstract
A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein, as a method for detection of the minute quantities of antigen and antibodies that are present in the tissues and body fluids of a bornaviraly infected cat. For this purpose reliable comparative assays, in the form of an ELISA and an IFA, had to be established. When this proved impossible within the time frame of the project, the aim of the study had to be abandoned, further underscoring the need for improved means of detec-tion of bornaviral antibodies and antigen.
| Main title: | PLA som metod för detektion av bornavirusinfektion hos katt |
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| Authors: | Lundqvist, Urban |
| Supervisor: | Berg, Mikael and Johansson Wensman, Jonas and Blomström, Anne-Lie |
| Examiner: | Bélak, Sándor |
| Series: | Examensarbete / Sveriges lantbruksuniversitet, Fakulteten för veterinärmedicin och husdjursvetenskap, Veterinärprogrammet |
| Volume/Sequential designation: | 2009:3 |
| Year of Publication: | 2009 |
| Level and depth descriptor: | Second cycle, A2E |
| Student's programme affiliation: | VY002 Veterinary Medicine Programme 330 HEC |
| Supervising department: | (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| Keywords: | Bornavirus, Katt, Vingelsjuka, Diagnostik, PLA |
| URN:NBN: | urn:nbn:se:slu:epsilon-6-61 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-6-61 |
| Subject. Use of subject categories until 2023-04-30.: | Animal diseases |
| Language: | Swedish |
| Deposited On: | 22 Sep 2009 11:27 |
| Metadata Last Modified: | 20 Apr 2012 14:09 |
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