Persson, Karl, 2012. Programmed cell death in the moss Physcomitrella patens : studying the role of metacaspases in archegonia development through gene knockout by homologous recombination. First cycle, G2E. Uppsala: SLU, Dept. of Plant Biology and Forest Genetics (until 131231)
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Abstract
The knowledge of programmed cell death (PCD) in plants is limited. The gene family of caspases in animals is known to be important for the execution of PCD. Putative homologues to caspases have been found in plants and are referred to as metacaspases. The model moss Physcomitrella patens is known to possess a high frequency of homologous recombination, which can be exploited for the generation of targeted gene knockout mutants. The female reproductive organ in P. patens shows rapid cell degradation during its maturation. This cell degradation is believed to be caused by PCD, and is therefore used as a model to study the process. A new metacaspase 5 knockout construct was successfully produced during this project, which, together with a metacaspase 3 construct received from a collaborator, was transformed into moss to generate two metacaspase single knockout lines and four double knockout lines.
| Main title: | Programmed cell death in the moss Physcomitrella patens |
|---|---|
| Subtitle: | studying the role of metacaspases in archegonia development through gene knockout by homologous recombination |
| Authors: | Persson, Karl |
| Supervisor: | Thelander, Mattias and Martin, Tom |
| Examiner: | Jens, Sundström |
| Series: | Examensarbete / Institutionen för växtbiologi och skogsgenetik, SLU |
| Volume/Sequential designation: | 130 |
| Year of Publication: | 2012 |
| Level and depth descriptor: | First cycle, G2E |
| Student's programme affiliation: | NK002 Biology with specialisation in Biotechnology - Bachelor's Programme, 180.0hp |
| Supervising department: | (NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231) |
| Keywords: | Gene technology, Biotechnology, Knockout, Reverse genetics, Homologous recombination, Cloning, Physcomitrella patens, Archegonia, Programmed cell death, metacaspases |
| URN:NBN: | urn:nbn:se:slu:epsilon-s-1652 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-1652 |
| Subject. Use of subject categories until 2023-04-30.: | Plant physiology - Growth and development Plant physiology - Reproduction |
| Language: | English |
| Deposited On: | 18 Sep 2012 11:59 |
| Metadata Last Modified: | 18 Sep 2012 12:00 |
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