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Wang, Jinhui, 2012. The effect of combining two biological control microbes on seed and root colonization. Second cycle, A2E. Uppsala: SLU, Dept. of Forest Mycology and Plant Pathology



Clonostachys rosea strain IK726 and Pseudomonas chlororaphis strain MA342 are two promising biocontrol microbes which both has various biocontrol mechanisms to control many seed and soil-borne plant diseases. Because a potential additive effect may exist between these two biocontrol microbes, like an expanded antagonistic spectrum and enhanced persistence of biocontrol effect, we expected to get a more competitive biocontrol with a mixture of IK726 and MA342. Strong colonization and high persistence ability on seed and root is one important trait of a potential biocontrol agent. This study was aimed to estimate the colonization ability on seed and root under the circumstances of joint and alone applications of IK726 and MA342 as seed-dressing on spring wheat cultivar Dacke. By comparing the dilution plating results of joint and alone inoculated treatments, we observed that IK726 slightly reduced the initial seed inoculum of MA342. But in the treatment with or without IK726, MA342 persisted a stable population density in root that is distinct from previous research. We got an unexpected result from dilution plating that IK726 has not been detected on the root of spring wheat which has been confirmed by PCR results. Mycelial growth and sporulation lagged behind root-tip elongation, and nutrient deficiency might be the causes of the poor performance of IK726 on spring wheat root.

Main title:The effect of combining two biological control microbes on seed and root colonization
Authors:Wang, Jinhui
Supervisor:Jensen, Dan and Hökeberg, Margareta and Karlsson, Magnus
Examiner:Elfstrand, Malin
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2012
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM008 Plant Biology - Master's Programme 120 HEC
Supervising department:(NL, NJ) > Dept. of Forest Mycology and Plant Pathology
Keywords:Biological control, Root Colonization, Clonostachys rosea, Pseudomonas chlororaphis
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Subject. Use of subject categories until 2023-04-30.:Plant diseases
Deposited On:21 Aug 2012 09:01
Metadata Last Modified:21 Aug 2012 09:01

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