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Maluping, Ramon, 2004. Potential aquatic bacterial pathogens in the Philippines and Thailand : studies on the occurrence, characterisation, detection of potential virulence determinants and antimicrobial susceptibility profile. Second cycle, A1N, A1F or AXX ( AXX). Uppsala: SLU, Dept. of Biomedical Sciences and Veterinary Public Health

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Abstract

Strains of Aeromonas spp., `non–cholera vibrios´(NCVs) and Plesiomonas shigelloides isolated from aquatic environments and fish and human diarrhoeal cases in the Philippines and Thailand were characterised for potential virulence
markers, such as the production of cytotoxin, cell-associated and cell-free haemolysin and their capacity to adhere to human intestinal (Henle 407) cells in
vitro. In addition, the occurrence of tlh and tdh haemolysin genes and urease activity among Vibrio parahaemolyticus strains were also investigated.

The antimicrobial susceptibility profile of these strains was identified with selected antibiotics used in human medicine and aquaculture. The minimum inhibitory concentration of ampicillin, ceftiofur, gentamicin, neomycin,streptomycin, enrofloxacin, florfenicol, oxytetracycline and trimethoprimsulphamethoxazole
was determined using broth microdilution method.

Susceptibility to imipenem and detection of extended-spectrum β-lactamases (ESBLs) in ampicillin resistant strains was achieved by the combination disc diffusion method.

The results showed that strains recovered from clinical sources (human and fish)produced the investigated potential virulence determinants, while these are absent in environmental strains. Such virulence factors may be involved in the pathogenesis of disease caused by these bacterial pathogens in both humans and animals.

Moreover, antimicrobial resistance among environmental strains was rare. Acquired resistance to tetracycline and fluoroquinolone was demonstrated in Aeromonas strains of clinical origin and it could be possible that acquired
resistance is associated with the use of antimicrobials in therapy.

Main title:Potential aquatic bacterial pathogens in the Philippines and Thailand
Subtitle:studies on the occurrence, characterisation, detection of potential virulence determinants and antimicrobial susceptibility profile
Authors:Maluping, Ramon
Supervisor:UNSPECIFIED
Examiner:UNSPECIFIED
Series:Report/International Master of Science programme, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences
Volume/Sequential designation:39
Year of Publication:2004
Level and depth descriptor:Second cycle, A1N, A1F or AXX
Student's programme affiliation:MSCVE Master of Science Programme in Veterinary Medicine 90 HEC
Department:(VH) > Dept. of Biomedical Sciences and Veterinary Public Health
Keywords:Aeromonas spp., Vibrio spp., P. shigelloides, Philippines, Thailand, virulence determinants, antimicrobial susceptibility
URN:NBN:urn:nbn:se:slu:epsilon-s-753
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-753
Subjects:Aquatic ecology
Language:English
Additional Information:Överförd från avhandlingsarkivet/Transferred from the Dissertations and Graduate Thesis Archive
Deposited On:21 Nov 2011 10:19
Metadata Last Modified:20 Apr 2012 14:23

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