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Wang, Dan, 2011. A genetic approach to identify raccoon dog within a large native meso-carnivore community. Second cycle, A2E. Umeå: SLU, Dept. of Wildlife, Fish and Environmental Studies

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Abstract

Introduced species often brings unforeseen and/or severe ecological impacts to ecosystem. These may be serving as vectors of disease, causing economic loss, ecosystem effects, reduction of biodiversity and interaction between species (e.g. predation, competition and introgression). So, it is important to do research about introduced species. Traditional research methods may be difficult when dealing with elusive introduced carnivores. Nowadays, non-invasive genetic sampling could be used to detect, identify and monitor invasive species. In this paper, I developed a protocol which uses 23 species specific primer pairs, based on mitochondrial DNA, to differentiate invasive raccoon dog and other native carnivores (European badger, red fox, pine marten, European otter, American mink, Eurasian lynx, domestic cat and domestic dog). PCR reactions were optimized by using muscle samples. Species identification requires amplification of one correct pair of primers
and amplification failure of all the other primer pairs. My work offers an effective solution for the identification of invasive and native carnivores. In the future, this protocol can be used to monitor carnivore community in a large area by using field samples.

Main title:A genetic approach to identify raccoon dog within a large native meso-carnivore community
Authors:Wang, Dan
Supervisor:Spong, Göran
Examiner:Thulin, Carl-Gustaf
Series:Examensarbete i biologi
Volume/Sequential designation:2011:7
Year of Publication:2011
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:SM003 Management of Fish and Wildlife Populations - Master's Programme 120 HEC
Department:(S) > Dept. of Wildlife, Fish and Environmental Studies
Keywords:raccoon dog, Nyctereutes procyonoides, PCR, carnivore, species specific primer
URN:NBN:urn:nbn:se:slu:epsilon-s-448
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-448
Subjects:Animal genetics and breeding
Animal ecology
Language:English
Deposited On:06 Jul 2011 12:51
Metadata Last Modified:20 Apr 2012 14:21

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