Gustafsson Schylander, Maximiliam, 2026. Characterization of Genomic and Coding Sequence Variation in the SKI2 Locus Following Whole Genome Duplication : identification and characterization of coding sequence and stop codon variation using MAFFT and comparative alignment. First cycle, G2E. Uppsala: SLU, Department of Plant Biology (from 140101)
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Abstract
This study investigated amino acid mutations in the SKI2 protein between diploid and tetraploid Mimulus plants following whole genome duplication (WGD) (See figure 1). By comparing protein sequences using multiple sequence alignment and analyzing predicted AlphaFold structures. 12 tetraploid-specific amino acid changes were found. Most changes were conservative and located in less functional regions, but three substitutions (S584Y, L918Q, and T1331M) stood out as strong candidates for potential functional changes.
| Main title: | Characterization of Genomic and Coding Sequence Variation in the SKI2 Locus Following Whole Genome Duplication |
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| Subtitle: | identification and characterization of coding sequence and stop codon variation using MAFFT and comparative alignment |
| Authors: | Gustafsson Schylander, Maximiliam |
| Supervisor: | Yant, Levi |
| Examiner: | Sicard, Adrien |
| Series: | Examensarbete / Institutionen för växtbiologi, SLU |
| Volume/Sequential designation: | 216 |
| Year of Publication: | 2026 |
| Level and depth descriptor: | First cycle, G2E |
| Student's programme affiliation: | Other |
| Supervising department: | (NL, NJ) > Department of Plant Biology (from 140101) |
| Keywords: | Whole genome duplication (WGD), polyploidy, adaptive mutations, RNA helicase, SK12 protein, protein structure |
| URN:NBN: | urn:nbn:se:slu:epsilon-s-22119 |
| Permanent URL: | http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-22119 |
| Language: | English |
| Deposited On: | 12 Jun 2026 08:11 |
| Metadata Last Modified: | 13 Jun 2026 01:00 |
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