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Hallström, Ida, 2022. Finding the physiological function of the enzyme GPCAT. : a gene expression study using Arabidopsis plants with silenced GPCAT gene. First cycle, G2E. Alnarp: SLU, Dept. of Plant Breeding (from 130101)



The aim of this project was to study the enzyme Glycerophosphocholine Acyltransferase (GPCAT) and try to find its physiological function. The biochemical reaction that the enzyme GPCAT catalyses is known, but its physiological function, e.g. why and when plants need the enzyme GPCAT, is unknown. To investigate the physiological function, Arabidopsis plants with (wild type) and without (knockout 19) the gene encoding for the enzyme GPCAT were cultivated and then exposed to abiotic stresses. The gene expression of six genes were studied by Q-RT-PCR and differences in gene expression was calculated by the Pfaffl method. The three genes that were associated with Sphingolipids (delta-9 acyl-lipid desaturase 2, very-long-chain 3-oxoacyl-CoA synthase and sphinganine C4-monooxygenase 1) showed higher gene expression in the knockout 19 plants than the wild type in the cold treatments. This implies that the silencing of GPCAT gene influences the genes in sphingolipids synthesis in the cold treated plants. Therefore, GPCAT might be involved in the regulation of lipids, with focus on sphingolipids, in cold stress.

Main title:Finding the physiological function of the enzyme GPCAT.
Subtitle:a gene expression study using Arabidopsis plants with silenced GPCAT gene
Authors:Hallström, Ida
Supervisor:Lager, Ida
Examiner:Grimberg, Åsa
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2022
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:LY003 Horticultural Science Programme 300 HEC
Supervising department:(LTJ, LTV) > Dept. of Plant Breeding (from 130101)
Keywords:enzyme GPCAT, gene expression, glycerophosphocholine acyltransferase, GPCAT, PC recycling pathway, physiological function, sphingolipids
Permanent URL:
Subject. Use of subject categories until 2023-04-30.:Plant genetics and breeding
Deposited On:29 Sep 2022 08:45
Metadata Last Modified:30 Sep 2022 01:01

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