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Dhakal, Rishap, 2022. Isolation, protocol optimization and screening of genotypes against net blotch and scald of barley in controlled condition. Second cycle, A2E. Alnarp: SLU, Dept. of Plant Breeding (from 130101)



Net blotch and scald are two economically important diseases of barley, causing significant losses
in yield in Nordic and Baltic states (NBS) countries and in Sweden. Identification and deployment
of resistant cultivars is the most effective method for controlling both the diseases. However,
response of cultivars towards the pathogen is a complex phenomenon. It depends on the genetics of
plants, environmental factors, isolates of pathogen, developmental stages of plants, and the
resistance source for the plant. Due to this complexity, assessment of the cultivars reaction in the
field requires consideration of multiple factors and along with that, has to face climate variability
every year. Therefore, in this work, we isolated both the disease-causing pathogen from Swedish
fields. A new optimized protocol was developed for culturing both pathogens in laboratory
conditions. Then, using one isolate each of the two pathogens, Swedish official trial cultivars and
breeding lines from NBS countries were tested for seedling-stage resistance against the two diseases
using the developed optimized protocol under controlled conditions. Afterwards, Best Linear
Unbiased Estimates (BLUEs) were used to categorize/rank the genotypes after scoring. Also, a rank
correlation analysis was used to compare the net blotch resistance of official trial cultivars under
controlled conditions and in a field experiment. 25 cultivars out of 37 showed similar resistance type
in both field and controlled condition. The results of this study provide data regarding the source of
resistance available in tested material and will help decision makers to recommend cultivars repleted
with resistance towards both the disease.

Main title:Isolation, protocol optimization and screening of genotypes against net blotch and scald of barley in controlled condition
Authors:Dhakal, Rishap
Supervisor:Chawade, Aakash and Mustafa, Zakieh
Examiner:Alexandersson, Erik
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2022
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:SM006 Plant Biology for Sustainable Production - Master's programme, 120.0hp
Supervising department:(LTJ, LTV) > Dept. of Plant Breeding (from 130101)
Keywords:barley, net blotch, scald, isolation, seedling resistance
Permanent URL:
Subject. Use of subject categories until 2023-04-30.:Plant diseases
Deposited On:09 Jun 2022 10:17
Metadata Last Modified:10 Jun 2022 01:00

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