Home About Browse Search
Svenska


Wen, Yaru, 2020. Characterization of MITF gene in crayfish and their possible role in innate immunity. Second cycle, A2E. Uppsala: SLU, Dept. of Animal Breeding and Genetics


Full text not available

Abstract

Microphthalmia-associated transcription factor (MITF) is a tissue-specific transcription factor (TF), with a basic helix-loop-helix leucine zipper (bHLH-LZ) domain, which binds to the canonical E-box sequence (5’-CANNTG-3’) in the promoter region of target genes.
In this study, the MITF-like protein was identified in the marbled crayfish (Procambarus virginalis). The full-length cDNA coding sequence of the most similar gene to MITF from marbled crayfish is 1284bp (427 amino acids). In the secondary and tertiary structure of the deduced amino acid sequence, a conserved functional structure of bHLH-LZ was shown, which could bind to E-box. In the phylogenetic analysis, this obtained MITF-like gene showed a lager evolutionary distance to all the vertebrates and some invertebrates like lancelet (Branchiostoma belcheri), starfish (Acanthaster planci), sea urchin (Paracentrotus lividus) and (Lytechinus variegatus), but is closer than worms (Caenorhabditis elegans). The bHLH-LZ domain is located in exon 6, exon 7, exon 8, and exon 9 in the MITF gene and has a similar structure as the corresponding exons in human. Besides, in this study, a comparison of exon-intron structure of MITF between human, mouse, fruit fly and marbled crayfish was performed, it was shown that the splicing sites of the bHLH-LZ domain in MITF gene might be conserved across species. To evaluate the possible role of MITF as a TF in innate immune system regulation, a similar prophenoloxidase (proPO) DNA sequence was analysed for the presence of E-box. The proPO gene is responsible for trapping and myelinization of pathogens in invertebrate. The proPO-like gene of marbled crayfish contains eight CANNTG sequences. In addition, anti-apoptotic factor (BCL-2)-like gene was found in marbled crayfish and four CANNTG sequences were found. Our results provided evidence of the presence of MITF-like gene in crayfish species and may provide knowledge on the role of MITF in innate immune activation.

Main title:Characterization of MITF gene in crayfish and their possible role in innate immunity
Authors:Wen, Yaru
Supervisor:Norouzitallab, Parisa and Andersson, Göran
Examiner:Mikko, Sofia
Series:UNSPECIFIED
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2020
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:VM006 Animal Science - Master's Programme
Supervising department:(VH) > Dept. of Animal Breeding and Genetics
Keywords:Microphthalmia-associated transcription factor, basic Helix-Loop-Helix Leucine Zipper, crayfish, Aphanomyces astaci, immunity
URN:NBN:urn:nbn:se:slu:epsilon-s-16418
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-16418
Subjects:Animal genetics and breeding
Language:English
Deposited On:12 Feb 2021 12:17
Metadata Last Modified:13 Feb 2021 02:01

Repository Staff Only: item control page

Downloads

Downloads per year (since September 2012)

View more statistics

Downloads
Hits