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Uddin, Mohammad Nasir, 2010. Characterisation of virus isolates associated with leaf yellowing in Swedish sugar-beet plants. Second cycle, A2E. Uppsala: SLU, Dept. of Plant Biology and Forest Genetics (until 131231)

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Abstract

Plants of sugar beet with symptoms of virus infection (mosaic and yellowing) were collected from Skåne in Sweden for virus identification and characterization. To test the presence of Beet mosaic virus (BtMV) several detection methods were applied, such as: mechanical inoculation/transmission test with sugar beet (Beta vulgaris), red beet (Beta vulgaris) cv Rubia, spinach (Spinacia oleracea) cv Long Standing Bloomsdale and lettuce (Lactuca sativa) cv Sonette; DAS-Enzyme-linked Immunosorbent Assay (DAS-ELISA) with polyclonal antibody; RT-PCR amplification with Potyviridae-specific universal primers Sprimer/M4 (Chen et al., 2001), and BtMV-specific primer pairs BM1/BM2 and BM1/BM3 (Glasa et al., 2003). No BtMV infection was observed from the results of the tests mentioned above. To check the possible infection of beet polero/luteovirus, RT-PCR amplification was conducted with Luteoviridae-specific universal primers Lu1/Lu4 (Robertson et al., 1991) and the expected band of ca 500 bp was found for the collected samples. The PCR fragments of the isolates were cloned, sequenced and then used for phylogenetic analyses. The sequence information of the two Swedish isolates SE1 and SE2 collected from Skåne revealed a sequence of 505 nucleotides and they were found to be 100% identical to each other. Comparison to the sequences in GenBank using BLASTn search showed 99% nucleotide identity and 100% amino acid identity (using BLASTp & BLASTx) with the Coat Protein encoding region of Beet mild yellowing virus (BMYV) isolates. Phylogenetic analyses with different evolutionary best-fit models showed that both of the isolates are very closely related to the British isolate BMYV-Broom’s Barn. The sequence information of isolate SE1 was submitted to GenBank and the accession number FN827048 was obtained. To our knowledge, this is the first molecular identification and sequence information for BMYV from Sweden.

Main title:Characterisation of virus isolates associated with leaf yellowing in Swedish sugar-beet plants
Authors:Uddin, Mohammad Nasir
Supervisor:Kvarnheden, Anders
Examiner:Kvarnheden, Anders
Series:Examensarbete / Sveriges lantbruksuniversitet, Institutionen för växtbiologi och skogsgenetik
Volume/Sequential designation:108
Year of Publication:2010
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:NM008 Plant Biology - Master's Programme 120 HEC
Department:(NL, NJ) > Dept. of Plant Biology and Forest Genetics (until 131231)
Keywords:BMYV, sugar beet, coat protein, BtMV, isolates, sequences, phylogenetic analyses
URN:NBN:urn:nbn:se:slu:epsilon-3-72
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-3-72
Subjects:Plant diseases
Language:English
Additional Information:The Msc thesis is a part of collaborated programme of Experimental Plant Biology among four universities: Uppsala University, SLU, Stockholm University and Södertörns Högskola, Sweden.
Deposited On:01 Jul 2010 13:05
Metadata Last Modified:20 Apr 2012 14:14

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