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Bäckström, Elin and Thalin, Therese, 2019. Evaluation of GM trees expressing a fungal enzyme : investigating the effects of the fungal enzyme on activation of jasmonate signaling using JAZ10:GUS reporter lines of Arabidopsis. First cycle, G2E. Umeå: SLU, Dept. of Forest Genetics and Plant Physiology

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Abstract

The fungi enzyme glucuronoyl esterase can be used to change the cell wall composition in plants for easier extraction of cellulosic biomass compounds for the production of biomaterials and biofuels. However, previous studies have shown that expression of glucuronoyl esterase in plants causes stress response, mediated by jasmonate signaling, and growth penalty. In this study, the GUS reporter system for jasmonate signaling was used to test if the transgenic plants react to the activity of glucuronoyl esterase or to the presence of foreign protein.

Arabidopsis line expressing JAZ10:GUS construct was used to test effects of glucuronoyl esterase applications on seven days old seedlings. The results obtained indicate that the fungal enzyme is recognized by the plant as pathogenic. The hypothesis that the activity contributes to the stress response cannot be rejected, therefore, further testing is needed to prove that. It was also concluded that the experiment require high number of plants samples for clear results, and that small disturbances of the seedlings can induce temporary stress response. Therefore, the time lag period between the selection of seedlings and the application of the enzyme should be at least 24 hours to avoid unwanted stress response.

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Svampenzymet glucuronoylesteras kan användas för att förändra cellväggskompositionen i växter för enklare extraktion av cellulosahaltiga biomassakomponenter, vilket är intressant för produktionen av olika biomaterial och biobränslen. Tidigare studier visar däremot att uttryck av glucuronoylesteras i plantor orsakar stress signalerad av jasmonater, samt förlorad tillväxt. I denna studie användes GUS-reportersystem för att undersöka om transgena Arabidopsisplantorna reagerar på enzymets aktivitet, eller känner igen enzymet som patogent.

En Arabidopsislinje som uttrycker JAZ10:GUS-konstruktion användes för att testa effekterna av glucuronoylesteras applicering på sju dagar gamla plantor. De erhållna resultaten indikerar att svampenzymet känns igen som en patogen, men hypotesen om att aktiviteten kan bidra till stressresponsen kan inte avvisas. Därför krävs ytterligare tester för att konfirmera eller utesluta aktivitetens påverkan. Det fastslogs också att experimentet kräver stor mängd plantor för att ge säkra resultat, samt att små störningar kring plantan kan inducera tillfällig stressrespons. Därför bör vilotiden mellan selektion och applikationen av substanserna vara minst 24 timmar, för att undvika oönskad stress.

Main title:Evaluation of GM trees expressing a fungal enzyme
Subtitle:investigating the effects of the fungal enzyme on activation of jasmonate signaling using JAZ10:GUS reporter lines of Arabidopsis
Authors:Bäckström, Elin and Thalin, Therese
Supervisor:Mellerowicz, Ewa
Examiner:Mörling, Tommy
Series:Kandidatarbeten i skogsvetenskap / SLU, Fakulteten för skogsvetenskap
Volume/Sequential designation:2019:3
Year of Publication:2019
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:SY001 Forest Science - Master's Programme 300 HEC
Supervising department:(S) > Dept. of Forest Genetics and Plant Physiology
Keywords:cell wall formation, cell wall composition, genetic modification, genetic improvement,, GUS
URN:NBN:urn:nbn:se:slu:epsilon-s-10708
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-10708
Subjects:Forestry - General aspects
Language:English
Deposited On:10 Jul 2019 13:16
Metadata Last Modified:11 Jul 2019 01:00

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