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Hellström, Maria, 2017. The use of cryoprotectants in unrooted cuttings of Pelargonium zonale, in order to increase their life expectancy. First cycle, G2E. Alnarp: SLU, Dept. of Biosystems and Technology (from 130101)



The rooting capacity of an unrooted Pelargonium zonale cutting decreases immensely 96 hours after being cut. The aim of this paper was to evaluate if two different cryoprotectants, 25% glucose and 30% trehalose, with the help of vacuum impregnation and pulse electric fields could result in an increase in the life expectancy of these cuttings. This was established by testing four different vacuum protocols were the average weight gain was noted. The optimal protocol was the first one where the average weight gain of the cutting was 17.13% when treated with glucose and was 24.75% when treated with trehalose. Pulsed electric fields parameters were optimised through trial and error were the optimum voltage was determined to be at 800V. Moreover, after treating the P. zonale cuttings with both vacuum impregnation and pulsed electric fields, they were stored at 6 ℃ for approximately 7 days and then they were planted in Jiffy-7 pots. This was repeated three times. Further, the root development was measured daily after approximately two weeks. The data was later analysed using a binary logistic regression where it illustrated that there was a significant difference between the untreated cuttings and the ones treated with 30% trehalose. A comparison between the two sugars conveyed that the cuttings treated with trehalose rooted better than those treated with glucose.


Målet med detta kandidatarbete var att testa och se om man kan utöka hållbarheten hos orotade Pelargonium zonale sticklingar med hjälp av vacuumimpregnering, puls elektriska fält och frysskyddsmedel. De två frysskyddsmedel som provades var 25% glukos och 30% trehalos. Fyra olika vacuum protokoll testades där vikt ökningen mättes. Det optimala protokollet visade sig vara det första där hela sticklingsvikten ökade med 17.13% när den behandlades med glukos, och med 24.75% när den behandlades med trehalos. Parametern för de puls elektriska fälten optimerades med hjälp av trial and error. Den optimala spänningen låg på 800V. Därefter, behandlades sticklingarna med både vacuumimpregnering samt puls elektriska fält. De behandlade sticklingarna plus några obehandlade förvarades i ungefär en vecka vid 6 ℃ i ett kylskåp. Därpå planterades de i Jiffy-7. Detta upprepades tre gånger. Rottillväxten mättes ca. två veckor efter planteringen. Det insamlade data analyserades med hjälp av en binär logistik regression där man såg en signifikant skillnad mellan de obehandlade och de som hade behandlads med trehalos. En jämförelse mellan de två behandlingarna visade att de sticklingarna som hade behandlads med trehalos hade rotad sig betydligt bättre än de som hade behandlads med glukos.

Main title:The use of cryoprotectants in unrooted cuttings of Pelargonium zonale, in order to increase their life expectancy
Authors:Hellström, Maria
Supervisor:Nordmark, Lotta and Demir, Eda
Examiner:Bergstrand, Karl-Johan
Volume/Sequential designation:UNSPECIFIED
Year of Publication:2017
Level and depth descriptor:First cycle, G2E
Student's programme affiliation:LY003 Horticultural Science Programme 300 HEC
Supervising department:(LTJ, LTV) > Dept. of Biosystems and Technology (from 130101)
Keywords:cryoprotectants, mother plants, Pelargonium cuttings, pulsed electric fields, vacuum impregnation, unrooted
Permanent URL:
Subject. Use of subject categories until 2023-04-30.:Plant ecology
Deposited On:15 Jan 2018 12:40
Metadata Last Modified:25 Feb 2019 13:14

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