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Mittl, Sabrina, 2017. Importance of sampling design using an eDNA monitoring approach for pond-living amphibians. Second cycle, A2E. Umeå: SLU, Dept. of Wildlife, Fish and Environmental Studies

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Abstract

The eDNA approach for monitoring aquatic species is already widely used. However, there are still uncertainties, for instance regarding the distribution of target DNA in ponds. These uncertainties need to be addressed when using eDNA in management. The current study uses individual capture and eDNA monitoring methods based on bottle trapping and 3x15 ml water sampling for Triturus cristatus in different ponds to show the importance of a carefully considered and performed study and in particular sampling design for achieving reliable results. Most of the results were received from only one pond. While here the bottle trapping resulted only in 15 catches, the eDNA method showed better results in confirming presence of the target. Examining two different primer combinations, the one with the bigger amplicon was more reliable than the one with the shorter amplicon. However, the data clearly indicated an influence of the sampling location on the eDNA results. This is also true for weather factors and / or sample time within the season. Therefore the sampling design has to be reconsidered, with respect to DNA distribution and persistence, seasonal habitation differences of the target and the primer specifics.

Main title:Importance of sampling design using an eDNA monitoring approach for pond-living amphibians
Authors:Mittl, Sabrina
Supervisor:Spong, Göran and Richter, Klaus
Examiner:Thulin, Carl-Gustaf
Series:Examensarbete i ämnet biologi / Sveriges lantbruksuniversitet, Institutionen för vilt, fisk och miljö
Volume/Sequential designation:2017:16
Year of Publication:2017
Level and depth descriptor:Second cycle, A2E
Student's programme affiliation:SM003 Management of Fish and Wildlife Populations - Master's Programme 120 HEC
Supervising department:(S) > Dept. of Wildlife, Fish and Environmental Studies
Keywords:eDNA, amphibians, non-invasive sampling, genetic monitoring
URN:NBN:urn:nbn:se:slu:epsilon-s-9375
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-9375
Subjects:Aquatic ecology
Language:English
Deposited On:13 Jun 2018 11:30
Metadata Last Modified:13 Jun 2018 11:30

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