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Sjöberg, Anna-Carin, 2005. Spermaadhesiner i olika fraktioner av hingstsperma. SLU, Dept. of Clinical Sciences (until 231231), Uppsala. Uppsala: SLU, Dept. of Clinical Sciences (until 231231)

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Abstract

Breeding-induced endometritis in horses seems to be down-regulated by certain components in the seminal plasma. These components are most likely some of the proteins in the seminal plasma in the stallion ejaculate, the so-called spermadhesins. This study aimed to recognize these proteins and determine their quantities in different fractions of the ejaculate. Fractionated ejaculates from four stallions were used to determine the presence and amount of proteins by way of size-excluding chromatography. All former known proteins, except two, were recognized in this study. There were no differences among fractions; neither within nor among stallions. Further studies need to be performed in order to study which specific protein or proteins seem to be the most important in down-regulating breeding-induced endometritis. These proteins should in that case be included in the insemination dose.

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Betäckningsinducerad endometrit hos sto verkar nedregleras av vissa komponenter i
seminalplasman i hingstens ejakulat. Dessa komponenter är sannolikt något eller
några av de proteiner som finns i seminalplasman i hingstens ejakulat, de så kallade
spermaadhesinerna. I denna studie undersöktes vilka dessa proteiner var och i vilken
mängd de fanns i olika fraktioner av ejakulatet. Fraktionerade ejakulat från fyra
hingstar användes för att bestämma vilka proteiner som fanns och hur stor deras
mängd var genom kromatografi som sorterade proteinerna enligt storlek. Alla tidigare
kända proteiner i seminalplasma hos hingst utom två hittades. Det fanns ingen
skillnad mellan fraktionerna; varken mellan eller inom hingstarna. Vidare studier
krävs för att undersöka vilket eller vilka specifika protein som är det/de viktigaste för
nedregleringen av betäckningsinducerad endometrit. Dessa protein bör i så fall ingå i
inseminationsdosen.

Main title:Spermaadhesiner i olika fraktioner av hingstsperma
Authors:Sjöberg, Anna-Carin
Supervisor:Tunon, Ann-Marie
Examiner:UNSPECIFIED
Series:Examensarbete / Sveriges lantbruksuniversitet, Fakulteten för veterinärmedicin och husdjursvetenskap, Veterinärprogrammet
Volume/Sequential designation:2005:48
Year of Publication:2005
Level and depth descriptor:Other
Student's programme affiliation:3050A Veterinary Medicine Programme (admitted before July 1, 2007) 330 HEC
Supervising department:(VH) > Dept. of Clinical Sciences (until 231231)
Keywords:spermaadhesiner, seminalplasma, hingstsperma, ejakulat, fraktioner
URN:NBN:urn:nbn:se:slu:epsilon-s-7873
Permanent URL:
http://urn.kb.se/resolve?urn=urn:nbn:se:slu:epsilon-s-7873
Subject. Use of subject categories until 2023-04-30.:Veterinary science and hygiene - General aspects
Faculties > Faculty of Veterinary Medicine and Animal Science
Language:Swedish
Deposited On:16 Oct 2017 11:38
Metadata Last Modified:16 Oct 2017 11:38

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